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Merck Supel™ Carbon LC Columns
商品簡述：
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高溫穩定性：可在高達 250°C 的溫度下操作，分離更快速且高效。
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pH 穩定性：在 1-14 pH 範圍內的流動相可兼容，且不影響柱壽命。
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獨特的保留機制：石墨的極性保留效應（PREG）使極性或帶電化合物在無需 HILIC 條件下得到保留，並能解析幾何異構物。
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溶劑兼容性：可使用任何極性或非極性溶劑。
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壓力穩定性：可承受高達 700 bar 的壓力。
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Supel™ Carbon LC Specifications |
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Particle Platform |
Porous Graphitic Carbon (PGC) |
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Particle Size |
2.7 µM |
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Pore Size |
200 Å |
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Surface Area |
155 m2/g |
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pH Range |
1-14 |
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Maximum |
250 °C |
The Supel™ Carbon LC columns are available in internal diameters of 2.1, 3.0, and 4.6 mm with guard cartridges and holders available for each inner diameter. 可兼容所有 HPLC、UHPLC、UPLC 和 LC-MS 儀器。
產品的應用
- 分析維他命Ｄ2維他命D3 Vitamin D2, Vitamin D3,
- N-Glycans from Human IgG,
- 分析20 種 Underivatized Amino Acids,
- 分析Polar Pesticides
檔案下載
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Supel™ Carbon LC 管柱
提供 2.1 mm、3.0 mm 和 4.6 mm 的內徑，並且每種內徑都有可用的保護管柱和holder。
Specifications
Particle Platform Porous Graphitic Carbon (PGC) Particle Size 2.7 µM Pore Size 200 Å Surface Area 155 m2/g pH Range 1-14 Maximum Temperature 250 °C 優 點
- Supel™ Carbon LC 管柱的合成採用專利的合成工藝，生成了窄的顆粒大小分佈（PSD）。此窄PSD減少了由渦流擴散效應引起的峰寬，從而提高了效率。
- 優異的解析能力: 應用在維生素 D2 和 維生素D3 代謝物的分離。這些代謝物極性大且結構相似，使它們在傳統矽膠基逆向層析管柱上難以分離。然而，Supel™ Carbon LC 管柱能以高效解析這些代謝物。
.Chromatographic separation of Vitamin D2 and D3 metabolites on Supel™ Carbon LC. Conditions: Column: Supel™ Carbon LC, 10 cm x 2.1 mm I.D., 2.7 µM; Mobile Phase: [A] 2-Propanol; [B] Tetrahydrofuran; Gradient: 0% B to 70% B in 15 min; hold at 70% B for 5 min; Flow Rate: 0.3 mL/min; Column Temp.: 25 °C; Detector: UV, 275 nm; Injection: 2.0 µL; Sample: Vitamin D2 and D3 metabolites mix, varied concentration, ethanol
Peaks Compound Retention Time (min) 1 3-epi-25-hydroxyvitamin D3 (50 µg/mL) 8.294 2 25-hydroxyvitamin D3 (25 µg/mL) 9.125 3 3-epi-25-hydroxyvitamin D2 (50 µg/mL) 11.126 4 25-hydroxyvitamin D2 (100 µg/mL) 12.052 Table 1.Peak Identities of Vitamin D2 and D3 Metabolites on Supel™ Carbon LC Column. - Supel™ Carbon LC 管柱能夠兼容所有 HPLC、UHPLC、UPLC 和 LC-MS 儀器
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Product No. Product Name Description 59997-U 2.7 μm HPLC Column 5 cm × 4.6 mm 59998-U 2.7 μm HPLC Column 10 cm × 4.6 mm 59991-U 2.7 μm HPLC Column 5 cm × 3.0 mm 59994-U 2.7 μm HPLC Column 15 cm × 3.0 mm 59984-U 2.7 μm HPLC Column 5 cm × 2.1 mm 59987-U 2.7 μm HPLC Column 15 cm × 2.1 mm 59995-U 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge , 2 cm × 4.0 mm 59988-U 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge, 2 cm × 3.0 mm 59981-U 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge, 2 cm × 2.1 mm 59996-U 2.7 μm HPLC Guard Cartridge Kit Kit, 2 cm × 4.0 mm 59989-U 2.7 μm HPLC Guard Cartridge Kit Kit, 2 cm × 3.0 mm 59982-U 2.7 μm HPLC Guard Cartridge Kit Kit, 2 cm × 2.1 mm 59999-U Supel™ Carbon LC
Guard Cartridge HolderStand-alone, for use with
Supel™ Carbon LC guard cartridges
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產品介紹
Supel™ Carbon LC 管柱
提供 2.1 mm、3.0 mm 和 4.6 mm 的內徑，並且每種內徑都有可用的保護管柱和holder。
Specifications
Particle Platform Porous Graphitic Carbon (PGC) Particle Size 2.7 µM Pore Size 200 Å Surface Area 155 m2/g pH Range 1-14 Maximum Temperature 250 °C 優 點
- Supel™ Carbon LC 管柱的合成採用專利的合成工藝，生成了窄的顆粒大小分佈（PSD）。此窄PSD減少了由渦流擴散效應引起的峰寬，從而提高了效率。
- 優異的解析能力: 應用在維生素 D2 和 維生素D3 代謝物的分離。這些代謝物極性大且結構相似，使它們在傳統矽膠基逆向層析管柱上難以分離。然而，Supel™ Carbon LC 管柱能以高效解析這些代謝物。
.Chromatographic separation of Vitamin D2 and D3 metabolites on Supel™ Carbon LC. Conditions: Column: Supel™ Carbon LC, 10 cm x 2.1 mm I.D., 2.7 µM; Mobile Phase: [A] 2-Propanol; [B] Tetrahydrofuran; Gradient: 0% B to 70% B in 15 min; hold at 70% B for 5 min; Flow Rate: 0.3 mL/min; Column Temp.: 25 °C; Detector: UV, 275 nm; Injection: 2.0 µL; Sample: Vitamin D2 and D3 metabolites mix, varied concentration, ethanol
Peaks Compound Retention Time (min) 1 3-epi-25-hydroxyvitamin D3 (50 µg/mL) 8.294 2 25-hydroxyvitamin D3 (25 µg/mL) 9.125 3 3-epi-25-hydroxyvitamin D2 (50 µg/mL) 11.126 4 25-hydroxyvitamin D2 (100 µg/mL) 12.052 Table 1.Peak Identities of Vitamin D2 and D3 Metabolites on Supel™ Carbon LC Column. - Supel™ Carbon LC 管柱能夠兼容所有 HPLC、UHPLC、UPLC 和 LC-MS 儀器
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產品規格
Product No. Product Name Description 59997-U 2.7 μm HPLC Column 5 cm × 4.6 mm 59998-U 2.7 μm HPLC Column 10 cm × 4.6 mm 59991-U 2.7 μm HPLC Column 5 cm × 3.0 mm 59994-U 2.7 μm HPLC Column 15 cm × 3.0 mm 59984-U 2.7 μm HPLC Column 5 cm × 2.1 mm 59987-U 2.7 μm HPLC Column 15 cm × 2.1 mm 59995-U 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge , 2 cm × 4.0 mm 59988-U 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge, 2 cm × 3.0 mm 59981-U 2.7 μm HPLC Guard Cartridge pack of 3 ea, cartridge, 2 cm × 2.1 mm 59996-U 2.7 μm HPLC Guard Cartridge Kit Kit, 2 cm × 4.0 mm 59989-U 2.7 μm HPLC Guard Cartridge Kit Kit, 2 cm × 3.0 mm 59982-U 2.7 μm HPLC Guard Cartridge Kit Kit, 2 cm × 2.1 mm 59999-U Supel™ Carbon LC
Guard Cartridge HolderStand-alone, for use with
Supel™ Carbon LC guard cartridges
